A technique for rapid isolation of macrophages from guinea-pig peritoneal exudates.
نویسندگان
چکیده
Preparations of pure macrophage suspensions may be required in the investigation of cellmediated immune reactions or for the preparation of antimacrophagic serum. Methods for the preparation of macrophage suspensions of high purity from peritoneal exudates are relatively tedious and result in small yields of cells. Bloom and Bennett (1966) and Turk and Polak (1967) describe a technique inwhich guinea-pigperitoneal exudates are incubated in Petri dishes at 37°C in an atmosphere containing 10% CO2 for four to 24 hours. Those cells which adhere to the glass surface and cannot be removed by vigorous shaking are released by treatment with a 1/5000 solution of ethylenediamine tetraacetic acid. They reported their cell preparations to contain 99-4% to 99-8% macrophages but yields were not given. This technique depends on the property of macrophages to stick firmly to a glass surface, which distinguishes them from lymphocytes. An albumin gradient sedimentation method described by Vallee, Hughes, and Gibson (1947) has been applied to the problem of isolating tumour cells from human peripheral blood (Roberts, Wayne, McGarth, McGrew, and Cole, 1958) and a modification was used for a similar purpose by Spriggs and Alexander (1960). We report here the use of a simple column of two layers of bovine serum albumin to effect differential sedimentation of guinea-pig peritoneal exudate cells.
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ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 23 9 شماره
صفحات -
تاریخ انتشار 1970